Contemporary methods of plant biotechnology have a wide application in the commercial production of ornamental plants worldwide. The culture in vitro, findings about physiological roles of phytohormones and molecular cloning techniques are the main elements of plant biotechnology which is focused on the improvement of agricultural production. This project refers to development and application of modern biotechnological methods in the production of ornamental plants. Along with classical research related to study of morphogenesis in vitro, the transformation with Agrobacterium vectors will be conducted in view of producing plants with improved characteristics, while special attention will be paid to development and application of cryobiological methods. Nutritive and hormonal factors of morphogenesis in vitro will be studied for the purpose of the efficient regeneration. These researches would standardize the protocols for micropropagation in Chrysanthemum, Impatiens, Iris and Viola, as well as somatic embryogenesis in the cultures Allium and Iris. The emergence of various viral diseases is a major problem in the conventional production of ornamental plants. For this reason, special attention in this project will be paid to the application of modern cryobiological methods and genetic transformations with aim of producing healthy planting material. Since viral infections increase respiration, causing oxidative stress, the project will focus on the clarification of the mechanism of induced resistance of plants to this type of biotic stress. The development of the methods for regeneration, genetic transformation, cryopreservation, as well as studying stress and other physiological processes will contribute to the improvement of protocols for obtaining healthy planting material of ornamental plants.

Heat stress (HS) represents one of the most important limiting factors to crop productivity worldwide. As a result of global warming, potato yield losses of 10-26% are predicted for region of Southeast Europe, including Serbia, in the next 30 years. These losses can be reduced on 5-11% with adaptation in production methods concerning use of heat tolerant cultivars. The scope of proposed project comprises development and application of protein markers for fast and efficient heat tolerance screening of potato cultivars. Our project proposes utilization of stress proteins related to HS-tolerance and heat-labile proteins as a protein markers. Results of protein marker quantitative analysis will be correlated with morphometric parameters and yield parameters under HS for each of examined genotypes using adequate statistical methods and an optimal combination of protein markets will be selected. Our expectations are that proposed project will result in biotechnological solution for protein marker assisted screening for heat tolerance in potato and identification of HS tolerant potato cultivars suitable for growing in Serbia under changed climate conditions.


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