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Institute for Biological Research "Siniša Stanković"
National Institute of the Republic of Serbia
University of Belgrade

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The Role of Cellular Glutathione Redox Cycle and Glutathione in the Regulation of Ileum Contractility

Redox homeostasis is driven by the ratio of the concentrations of cellular redox couples. The aim of this study was to reduce 2GSH/GSSG turnover in an ex vivo ileum by the irreversible inhibition of glutathione reductase (GR) activity (by BCNU) and evaluate its effects on contractility, antioxidant enzyme activity, and thiol levels. Increasing concentrations of BCNU as well as a single EC50 BCNU dose significantly reduced both contraction amplitude and GR activity. The addition of cumulative doses...

In Vitro Propagation as a Strategy for Improving Phytochemical Production and Supporting Conservation of Tunisian Lavandula stoechas

Lavandula stoechas (Lamiaceae) is a highly valued medicinal plant due to its rich content of bioactive secondary metabolites, particularly rosmarinic acid. This study aimed to develop an efficient in vitro propagation protocol from seeds and to evaluate the influence of 6-benzylaminopurine (BAP) on morphogenesis and phenolic compound production while comparing the antioxidant and antifungal activities of methanolic extracts from in vitro cultured and wild-growing plants. Seeds were germinated...

Bioactive potential of Cota tinctoria – chemical composition, antimicrobial, antibiofilm and cytotoxic properties

Cota tinctoria, yellow chamomile, is underexplored plant in the terms of its bioactivity. The aim of this study was to evaluate bioactive potential of different ethanolic, hydroethanolic and aqueous extracts, precisely their antimicrobial, antibiofilm, and cytotoxic capacity, and to provide their chemical characterization. The highest extraction yield was achieved by extracting plant material with 30% and 50% ethanol. Total flavonoid content was highest in infusion aqueous extract. Dominant phytochemicals...

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